In the annoying dick-swinging competition that are the comments-board, someone asserted Pakistanis have a lot of steppe even on the maternal side. Really?
We have Sintashta mtDNA and the discordance was shocking to me. But there are some groups in Pakistan with detectable Sintashta mtDNA. These samples from Hazara, Kho, Pashtun, Kashmiri,and Kalash. They identify 8.4% steppe mtDNA. Pakistan as a whole has a lot more “West Eurasian” mtDNA, but that’s obviously due to the legacy of the IVC. Anyway, Complete mitogenomes document substantial genetic contribution from the Eurasian Steppe into northern Pakistani Indo-Iranian speakers:
In summary, based on available archeological and high-resolution mitogenome data from northwestern Pakistan, especially from Iranian and Dardic populations, who are suggested to be the surviving traces of early Indo-Iranian groups, we identified the genetic contributions of different dispersals from west Eurasia into northern Pakistan during the Bronze Age onward. Importantly, we identified five haplogroups as the genetic legacy of IE speakers from the Eurasian Steppe, likely dispersed along with the migration of IE-speaking populations during the Bronze Age into northern Pakistan, thus implying that IE language expansion into South Asia was not simply mediated by cultural diffusion. This migration contributed 8.4% of the gene pool of northern Pakistani IE speakers, suggesting this demographic connection, which is a possible source of IE language diffusion, could be one part of the complex demographic history of the region. Our results also provide implications on the two main hypotheses of IE language origination, viz. Anatolia and Steppe hypotheses. Considering that Steppe components were observed in all Indo-Iranian groups in northern Pakistan in our study, as well as in other regions in South Asia , while lineages possibly representing the genetic legacy of Neolithic farmers, e.g., R2e, K1, were either absent or not found in all of the IE-speaking groups in northern Pakistan, our results lend more support to the Steppe hypothesis, at least from a matrilineal perspective. Furthermore, these IE speakers, as evidenced by the genetic legacy identified here, also moved southward and contributed genetically, though to a rather limited extent, to the Indian subcontinent, suggesting northern Pakistan as a corridor in the spread of IE languages during the Bronze Age dispersals into South Asia. Since our study is only based on mtDNA data, which only reflect maternal histories of populations, more investigations based on genome-wide data are also needed to intensively dissect the expansion of IE speakers into South Asia.